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Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action. © 2024. The Author(s).

Citation

Jingyu Wang, Jinzhao Ji, Yongcheng Zhong, Wenxin Meng, Shaobin Wan, Xiaoqing Ding, Zihan Chen, Weiyong Wu, Kun Jia, Shoujun Li. Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs. Veterinary research. 2024 Mar 16;55(1):33

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PMID: 38493160

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