Jingyu Wang, Jinzhao Ji, Yongcheng Zhong, Wenxin Meng, Shaobin Wan, Xiaoqing Ding, Zihan Chen, Weiyong Wu, Kun Jia, Shoujun Li
Veterinary research 2024 Mar 16Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action. © 2024. The Author(s).
Jingyu Wang, Jinzhao Ji, Yongcheng Zhong, Wenxin Meng, Shaobin Wan, Xiaoqing Ding, Zihan Chen, Weiyong Wu, Kun Jia, Shoujun Li. Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs. Veterinary research. 2024 Mar 16;55(1):33
PMID: 38493160
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