CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis.

Lianyi Sun, Fengzhi Li, Shuwei Bai, Chunchao Bi

International ophthalmology 2024 Mar 17

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The purpose of this study was to investigate the effect of circ_HLCS on age-related cataract (ARC). Circ_HLCS, microRNA (miR)-338-3p, and bisphosphate 3'-nucleotidase 1 (BPNT1) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell proliferation and cell viability were assessed by the 5-Ethynyl-2'-deoxyuridinr and cell counting kit-8 assays. Cell apoptosis was detected by flow cytometry. Targeted correlations among circ_HLCS, miR-338-3p, and BPNT1 were verified by the dual-luciferase reporter and RNA pull-down assays. circ_HLCS was diminished in ARC tissues and UV-treated SRA01/04 cells. Elevated content of circ_HLCS undermined UV-induced cell proliferation inhibition and apoptosis. Mechanistically, circ_HLCS directly targeted miR-338-3p, and circ_HLCS regulated BPNT1 expression through miR-338-3p. Furthermore, reduction of miR-338-3p ameliorated UV-induced SRA01/04 cell damage by increasing BPNT1 expression. Taken together, these data suggested that circ_HLCS inhibited apoptosis of UV-treated SRA01/04 cells by miR-338-3p/BPNT1 axis. Therefore, circ_HLCS might be a potential therapeutic target for ARC. © 2024. The Author(s), under exclusive licence to Springer Nature B.V.

Citation

Lianyi Sun, Fengzhi Li, Shuwei Bai, Chunchao Bi. CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis. International ophthalmology. 2024 Mar 17;44(1):142