Sandwich-type aptamer-based biosensors for thrombin detection.

Somayeh Sahraneshin Samani, Elham Sameiyan, Farideh Tabatabaei Yazdi, Sayed Ali Mortazavi, Mona Alibolandi, Mohammad Ramezani, Seyed Mohammad Taghdisi, Khalil Abnous

Analytical methods : advancing methods and applications 2024 Apr 04

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Thrombin, a proteolytic enzyme, plays an essential role in catalyzing many blood clotting reactions. Thrombin can act as a marker for some blood-related diseases, such as leukemia, thrombosis, Alzheimer's disease and liver disease. Therefore, its diagnosis is of great importance in the fields of biological and medical research. Biosensors containing sandwich-type structures have attracted much consideration owing to their superior features such as reproducible and stable responses with easy improvement in the sensitivity of detection. Sandwich-type platforms can be designed using a pair of receptors that are able to bind to diverse locations of the same target. Herein, we investigate recent advances in the progress and applications of thrombin aptasensors containing a sandwich-type structure, in which two thrombin-binding aptamers (TBAs) identify different parts of the thrombin molecule, leading to the formation of a sandwich structure and ultimately signal detection. We also discuss the pros and cons of these approaches and outline the most logical approach in each section.

Citation

Somayeh Sahraneshin Samani, Elham Sameiyan, Farideh Tabatabaei Yazdi, Sayed Ali Mortazavi, Mona Alibolandi, Mohammad Ramezani, Seyed Mohammad Taghdisi, Khalil Abnous. Sandwich-type aptamer-based biosensors for thrombin detection. Analytical methods : advancing methods and applications. 2024 Apr 04;16(14):1985-2001