Correlation Engine 2.0
Clear Search sequence regions


  • adult (1)
  • flounder (6)
  • myoblasts (2)
  • rna (4)
  • rna circular (2)
  • skeletal muscle (6)
  • Sizes of these terms reflect their relevance to your search.

    Circular RNAs (circRNAs) are non-coding RNAs with endogenous regulatory functions, including regulating skeletal muscle development. However, its role in the development of skeletal muscle in Japanese flounder (Paralichthys olivaceus) is not clear. Therefore we screened a candidate circpdlim5a, which is derived from the gene pdlim5a, from the skeletal muscle transcriptome of Japanese flounder. We characterized circpdlim5a, which was more stable compared to the linear RNA pdlim5a. Distributional characterization of circpdlim5a showed that circpdlim5a was predominantly distributed in the nucleus and was highly expressed in the skeletal muscle of adult Japanese flounder (24 months). When we further studied the circpdlim5a function, we found that it inhibited the expression of proliferation and differentiation genes according to the over-expression experiment of circpdlim5a in myoblasts. We concluded that circpdlim5a may inhibit the proliferation and differentiation of myoblasts and thereby inhibit skeletal muscle development in Japanese flounder. This experiment provides information for the study of circRNAs by identifying circpdlim5a and exploring its function, and offers clues for molecular breeding from an epigenetic perspective. Copyright © 2024 Elsevier Inc. All rights reserved.

    Citation

    Hao Wang, Min Liu, Hengtai Tang, Zhirui Zhang, Haishen Wen, Feng He. Identification and functional analysis of circpdlim5a generated from pdlim5a gene splicing in the skeletal muscle of Japanese flounder (Paralichthys olivaceus). General and comparative endocrinology. 2024 Jun 01;352:114500

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 38508470

    View Full Text