Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China.

The occurrence of geminiviruses causes significant economic losses in many economically important crops. In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames. Phylogenetic and genome-wide pairwise identity analysis revealed that TLCCxV shared less than 91% identities with reported geminiviruses. A TLCCxV infectious clone was constructed and successfully infected Nicotiana benthamiana, N. tabacum, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants. Furthermore, expression of the V2, C1 and C4 proteins through a potato virus X vector caused severe chlorosis or necrosis symptom in N. benthamiana. Taken together, we identified a new geminivirus in tobacco plants, and found that V2, C1 and C4 contribute to symptom development. Copyright © 2024 Elsevier Inc. All rights reserved.

Citation

Yuan Chen, Shiping Guo, Lianqiang Jiang, Fangfang Yan, Kaiqiang Hao, Zhiping Wang, Mengnan An, Zihao Xia, Fangfang Li, Xueping Zhou, Yuanhua Wu. Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China. Virology. 2024 Jun;594:110061

Mesh Tags

PMID: 38518441

search → result