Hannes Maib, Petia Adarska, Robert Hunton, James H Vines, David Strutt, Francesca Bottanelli, David H Murray
The Journal of cell biology 2024 Jun 03Phosphoinositides are a small family of phospholipids that act as signaling hubs and key regulators of cellular function. Detecting their subcellular distribution is crucial to gain insights into membrane organization and is commonly done by the overexpression of biosensors. However, this leads to cellular perturbations and is challenging in systems that cannot be transfected. Here, we present a toolkit for the reliable, fast, multiplex, and super-resolution detection of phosphoinositides in fixed cells and tissue, based on recombinant biosensors with self-labeling SNAP tags. These are highly specific and reliably visualize the subcellular distributions of phosphoinositides across scales, from 2D or 3D cell culture to Drosophila tissue. Further, these probes enable super-resolution approaches, and using STED microscopy, we reveal the nanoscale organization of PI(3)P on endosomes and PI(4)P on the Golgi. Finally, multiplex staining reveals an unexpected presence of PI(3,5)P2-positive membranes in swollen lysosomes following PIKfyve inhibition. This approach enables the versatile, high-resolution visualization of multiple phosphoinositide species in an unprecedented manner. © 2024 Maib et al.
Hannes Maib, Petia Adarska, Robert Hunton, James H Vines, David Strutt, Francesca Bottanelli, David H Murray. Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides. The Journal of cell biology. 2024 Jun 03;223(6)
PMID: 38578646
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