A Highly Sensitive and Rapid Colloidal Gold Immunoassay for Puerarin Detection.

Shiyan Yue, Yunxiang Liu, Rongrong Zhou, Zhilai Zhan, Liping Kang, Luqi Huang, Yuan Yuan, Tiegui Nan

Journal of agricultural and food chemistry 2024 Apr 17

filter terms:

Radix Puerariae is a traditional Chinese medicinal material with a rich history of use in East and Southeast Asia. Puerarin, a unique component of the Pueraria genus, serves as a quality control marker for herbal medicines like Pueraria lobata and Pueraria thomsonii in China, displaying diverse pharmacological properties. This study developed puerarin colloidal gold immunoassay dipsticks utilizing an anti-puerarin monoclonal antibody, resulting in a fast and sensitive detection method with a limit of 500-1000 ng·mL-1. Evaluation using tap water-extracted P. lobata and P. thomsonii samples showed consistent results compared to LC-MS analysis. Cross-reactivity assessments of puerarin analogs revealed minimal interference, affirming the dipstick's reliability for distinguishing between the two species.

Citation

Shiyan Yue, Yunxiang Liu, Rongrong Zhou, Zhilai Zhan, Liping Kang, Luqi Huang, Yuan Yuan, Tiegui Nan. A Highly Sensitive and Rapid Colloidal Gold Immunoassay for Puerarin Detection. Journal of agricultural and food chemistry. 2024 Apr 17;72(15):8817-8822