Yu Mu, Shan Zhao, Jing Liu, Zheyi Liu, Jian He, Hongfang Cao, Heng Zhao, Caiyun Wang, Yan Jin, Yanxia Qi, Fangjun Wang
Journal of agricultural and food chemistry 2024 May 01Lactoferrin (LTF) has diverse biological activities and is widely used in functional foods and active additives. Nevertheless, evaluating the proteoform heterogeneity, conformational stability, and activity of LTF remains challenging during its production and storage processes. In this study, we describe the implementation of native mass spectrometry (nMS), glycoproteomics, and an antimicrobial activity assay to assess the quality of LTF. We systematically characterize the purity, glycosylation heterogeneity, conformation, and thermal stability of LTF samples from different sources and transient high-temperature treatments by using nMS and glycoproteomics. Meanwhile, the nMS peak intensity and antimicrobial activity of LTF samples after heat treatment decreased significantly, and the two values were positively correlated. The nMS results provide essential molecular insights into the conformational stability and glycosylation heterogeneity of different LTF samples. Our results underscore the great potential of nMS for LTF quality control and activity evaluation in industrial production.
Yu Mu, Shan Zhao, Jing Liu, Zheyi Liu, Jian He, Hongfang Cao, Heng Zhao, Caiyun Wang, Yan Jin, Yanxia Qi, Fangjun Wang. Assessment of the Conformation Stability and Glycosylation Heterogeneity of Lactoferrin by Native Mass Spectrometry. Journal of agricultural and food chemistry. 2024 May 01;72(17):10089-10096
PMID: 38626386
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