Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics.

Mend Amar Batbaatar, Takeshi Kinoshita, Shinya Ikeda, Kiyoto Nishi, Hirotaka Iwasaki, Narangerel Ganbaatar, Mikiko Ohno, Eiichiro Nishi

Biochemical and biophysical research communications 2024 Jun 18

filter terms:

Blood pressure is a crucial physiological parameter and its abnormalities can cause a variety of health problems. We have previously reported that mice with systemic deletion of nardilysin (NRDC), an M16 family metalloprotease, exhibit hypotension. In this study, we aimed to clarify the role of NRDC in vascular smooth muscle cell (VSMC) by generating VSMC-specific Nrdc knockout (VSMC-KO) mice. Our findings reveal that VSMC-KO mice also exhibit hypotension. Aortas isolated from VSMC-KO mice exhibited a weakened contractile response to phenylephrine, accompanied by reduced phosphorylation of myosin light chain 2 and decreased rhoA expression. VSMC isolated from VSMC-KO aortas showed a reduced increase in intracellular Ca2+ concentration induced by α-stimulants. These findings suggest that NRDC in VSMC regulates vascular contraction and blood pressure by modulating Ca2+ dynamics. Copyright © 2024 Elsevier Inc. All rights reserved.

Citation

Mend Amar Batbaatar, Takeshi Kinoshita, Shinya Ikeda, Kiyoto Nishi, Hirotaka Iwasaki, Narangerel Ganbaatar, Mikiko Ohno, Eiichiro Nishi. Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics. Biochemical and biophysical research communications. 2024 Jun 18;712-713:149961