Yuimi Koyama, Mary Ann Suico, Aimi Owaki, Ryoichi Sato, Jun Kuwazuru, Shota Kaseda, Yuya Sannomiya, Jun Horizono, Kohei Omachi, Tomoko Horinouchi, Tomohiko Yamamura, Haruki Tsuhako, Kandai Nozu, Tsuyoshi Shuto, Hirofumi Kai
Clinical and experimental nephrology 2024 SepAlport syndrome (AS) is a genetic kidney disease caused by a mutation in type IV collagen α3, α4, and α5, which are normally secreted as heterotrimer α345(IV). Nonsense mutation in these genes causes severe AS phenotype. We previously revealed that the exon-skipping approach to remove a nonsense mutation in α5(IV) ameliorated the AS pathology. However, the effect of removing an exon on trimerization is unknown. Here, we assessed the impact of exon deletion on trimerization to evaluate their possible therapeutic applicability and to predict the severity of mutations associated with exon-skipping. We produced exon deletion constructs (ΔExon), nonsense, and missense mutants by mutagenesis and evaluated their trimer formation and secretion activities using a nanoluciferase-based assay that we previously developed. Exon-skipping had differential effects on the trimer secretion of α345(IV). Some ΔExons could form and secrete α345(IV) trimers and had higher activity compared with nonsense mutants. Other ΔExons had low secretion activity, especially for those with exon deletion near the C-terminal end although the intracellular trimerization was normal. No difference was noted in the secretion of missense mutants and their ΔExon counterpart. Exon skipping is advantageous for nonsense mutants in AS with severe phenotypes and early onset of renal failure but applications may be limited to ΔExons capable of normal trimerization and secretion. This study provides information on α5(IV) exon-skipping for possible therapeutic application and the prediction of the trimer behavior associated with exon-skipping in Alport syndrome. © 2024. The Author(s), under exclusive licence to Japanese Society of Nephrology.
Yuimi Koyama, Mary Ann Suico, Aimi Owaki, Ryoichi Sato, Jun Kuwazuru, Shota Kaseda, Yuya Sannomiya, Jun Horizono, Kohei Omachi, Tomoko Horinouchi, Tomohiko Yamamura, Haruki Tsuhako, Kandai Nozu, Tsuyoshi Shuto, Hirofumi Kai. Trimerization profile of type IV collagen COL4A5 exon deletion in X-linked Alport syndrome. Clinical and experimental nephrology. 2024 Sep;28(9):874-881
PMID: 38658441
View Full Text