Genome-wide identification and analysis of DEAD-box RNA helicases in Gossypium hirsutum.

DEAD-box RNA helicases, a prominent subfamily within the RNA helicase superfamily 2 (SF2), play crucial roles in the growth, development, and abiotic stress responses of plants. This study identifies 146 DEAD-box RNA helicase genes (GhDEADs) and categorizes them into four Clades (Clade A-D) through phylogenetic analysis. Promoter analysis reveals cis-acting elements linked to plant responses to light, methyl jasmonate (MeJA), abscisic acid (ABA), low temperature, and drought. RNA-seq data demonstrate that Clade C GhDEADs exhibit elevated and ubiquitous expression across different tissues, validating their connection to leaf development through real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Notably, over half of GhDEADs display up-regulation in the leaves of virus-induced gene silencing (VIGS) plants of GhVIR-A/D (members of m6A methyltransferase complex, which regulate leaf morphogenesis). In conclusion, this study offers a comprehensive insight into GhDEADs, emphasizing their potential involvement in leaf development. Copyright © 2024 Elsevier B.V. All rights reserved.

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Ruotong Rao, Xiaoyu Huang, Xinting Wang, Xuelong Li, Huiping Liao, Nigara Abuduwaili, Xiuzhen Wei, Dengdi Li, Gengqing Huang. Genome-wide identification and analysis of DEAD-box RNA helicases in Gossypium hirsutum. Gene. 2024 Aug 20;920:148495

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PMID: 38663690

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