Structural elucidation of the mesothelin-mucin-16/CA125 interaction.

Mesothelin (MSLN) is a cell-surface glycoprotein expressed at low levels on normal mesothelium but overexpressed in many cancers. Mesothelin has been implicated to play role/s in cell adhesion and multiple signaling pathways. Mucin-16/CA125 is an enormous cell-surface glycoprotein, also normally expressed on mesothelium and implicated in the progression and metastasis of several cancers, and directly binds mesothelin. However, the precise biological function/s of mesothelin and mucin-16/CA125 remain mysterious. We report protein engineering and recombinant production, qualitative and quantitative binding studies, and a crystal structure determination elucidating the molecular-level details governing recognition of mesothelin by mucin-16/CA125. The interface is small, consistent with the ∼micromolar binding constant and is free of glycan-mediated interactions. Sequence comparisons and modeling suggest that multiple mucin-16/CA125 modules can interact with mesothelin through comparable interactions, potentially generating a high degree of avidity at the cell surface to overcome the weak affinity, with implications for functioning and therapeutic interventions. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Peter B Rupert, Matthew Buerger, Della J Friend, Roland K Strong. Structural elucidation of the mesothelin-mucin-16/CA125 interaction. Structure (London, England : 1993). 2024 Aug 08;32(8):1049-1054.e2

Mesh Tags

Substances

PMID: 38703776

search → result