Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels.

The development of cell-based fluorescent assays has resulted in an incredible tool for searching new ion channels' modulators with a biophysical and clinical profile. Among all the ion channels, potassium (K+)-permeable channels represent the most diverse and relevant for cell function, making them attractive targets for drug discovery. Some of the cell-based assays for K+ channels take advantage of a thallium-sensitive dye whose fluorescence increased upon the binding of thallium (Tl+), an ion able to move through K+ channels. We optimize the FLIPR Potassium Assay Kit based on thallium influx to measure the Kv10.1 activity. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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María Luisa Durán-Pastén, Enoch Luis. Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels. Methods in molecular biology (Clifton, N.J.). 2024;2796:97-103

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PMID: 38856897

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