Correlation Engine 2.0
Clear Search sequence regions


  • genes (1)
  • mice (3)
  • muscle (5)
  • muscle skeletal (1)
  • MuSCs (2)
  • MYF5 (5)
  • PAX7 (7)
  • self (4)
  • stem cell (7)
  • Sizes of these terms reflect their relevance to your search.

    Increasing evidence suggests that the muscle stem cell (MuSC) pool is heterogeneous. In particular, a rare subset of PAX7-positive MuSCs that has never expressed the myogenic regulatory factor MYF5 displays unique self-renewal and engraftment characteristics. However, the scarcity and limited availability of protein markers make the characterization of these cells challenging. Here, we describe the generation of StemRep reporter mice enabling the monitoring of PAX7 and MYF5 proteins based on equimolar levels of dual nuclear fluorescence. High levels of PAX7 protein and low levels of MYF5 delineate a deeply quiescent MuSC subpopulation with an increased capacity for asymmetric division and distinct dynamics of activation, proliferation, and commitment. Aging primarily reduces the MYF5Low MuSCs and skews the stem cell pool toward MYF5High cells with lower quiescence and self-renewal potential. Altogether, we establish the StemRep model as a versatile tool to study MuSC heterogeneity and broaden our understanding of mechanisms regulating MuSC quiescence and self-renewal in homeostatic, regenerating, and aged muscles. Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

    Citation

    Sara Ancel, Joris Michaud, Federico Sizzano, Loic Tauzin, Manuel Oliveira, Eugenia Migliavacca, Svenja C Schüler, Sruthi Raja, Gabriele Dammone, Sonia Karaz, José L Sánchez-García, Sylviane Metairon, Guillaume Jacot, C Florian Bentzinger, Jérôme N Feige, Pascal Stuelsatz. A dual-color PAX7 and MYF5 in vivo reporter to investigate muscle stem cell heterogeneity in regeneration and aging. Stem cell reports. 2024 Jul 09;19(7):1024-1040

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 38876109

    View Full Text