Protocol for the collection and analysis of the different immune cell subsets in the murine intestinal lamina propria.

The intestinal lamina propria (LP) is a leukocyte-rich cornerstone of the immune system owing to its vital role in immune surveillance and barrier defense against external pathogens. Here, we present a protocol for isolating and analyzing immune cell subsets from the mouse intestinal LP for further downstream applications. Starting from tissue collection and cleaning, epithelium removal, and enzymatic digestion to collection of single cells, we explain each step in detail to maximize the yield of immune cells from the intestinal LP. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Arthi Shanmugavadivu, Katlynn Carter, Aysan Poursadegh Zonouzi, Ari Waisman, Tommy Regen. Protocol for the collection and analysis of the different immune cell subsets in the murine intestinal lamina propria. STAR protocols. 2024 Sep 20;5(3):103154

Mesh Tags

PMID: 38935510

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