Belimumab concentration measurements using a homologous anti-idiotype immunoassay.

Journal of immunological methods 2024 Sep

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Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations. Copyright © 2024 Elsevier B.V. All rights reserved.

Citation

Floris C Loeff, Ioannis Parodis, Tomas Walhelm, Andreas Jönsen, Dionysis Nikolopoulos, Christopher Sjöwall, Anders A Bengtsson, Dorien Kos, Astrid van Leeuwen, Bryan van den Broek, Lisanne Dijk, Jorn Jeremiasse, Birgit S Blomjous, Alexandre E Voskuyl, Gerrit Jan Wolbink, Irene E M Bultink, Theo Rispens. Belimumab concentration measurements using a homologous anti-idiotype immunoassay. Journal of immunological methods. 2024 Sep;532:113717