Optimizing the preparation of paraffin sections from stallion testes.

The preparation of paraffin sections is an important experimental technique in animal histological research, and key factors that determine the quality of a section include the dehydration time, waxing time, and drying temperature of the paraffin section. Paraffin sections obtained from testis tissue of adult horses exhibited higher quality with clear tissue structure and complete cell morphology after they underwent gradient dehydration for 6 hours, were immersed in wax for 60 minutes, and were dried in a 75-degree oven for 15 minutes. The detailed, optimized procedures that are developed in the current study may simplify histological experiments and research on equine testes. ©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.

Citation

Nairag Asgenbaatar, Minna Yi, Xisheng Wang, Tseweendolmaa Ulaangerel, Yingchao Shen, Xin Wen, Ming Du, Xiaoling Dong, Yibeeltu Mengkh, Manglai Dugarjav, Gerelchimeg Bou. Optimizing the preparation of paraffin sections from stallion testes. Histology and histopathology. 2024 Jun 06:18775

PMID: 38984371

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