A bifunctional Pasteurella multocida β1-3-galactosyl/N-acetylgalactosaminyltransferase (PmNatB) for the highly efficient chemoenzymatic synthesis of disaccharides.

Xiaohong Yang, Bijoyananda Mishra, Hai Yu, Yijun Wei, Xi Chen

Organic & biomolecular chemistry 2024 Jul 24

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Glycosyltransferases are nature's key biocatalysts for the formation of glycosidic bonds. Discovery and characterization of new synthetically useful glycosyltransferases are critical for the development of efficient enzymatic and chemoenzymatic strategies for producing complex carbohydrates and glycoconjugates. Herein we report the identification of Pasteurella multocida PmNatB as a bifunctional single-catalytic-domain glycosyltransferase with both β1-3-galactosyltransferase and β1-3-N-acetylgalactosaminyltransferase activities. It is a novel glycosyltransferase for constructing structurally diverse GalNAcβ3Galα/βOR and Galβ3GalNAcα/βOR disaccharides in one-pot multienzyme systems with in situ generation of UDP-sugars.

Citation

Xiaohong Yang, Bijoyananda Mishra, Hai Yu, Yijun Wei, Xi Chen. A bifunctional Pasteurella multocida β1-3-galactosyl/N-acetylgalactosaminyltransferase (PmNatB) for the highly efficient chemoenzymatic synthesis of disaccharides. Organic & biomolecular chemistry. 2024 Jul 24;22(29):6004-6015