Zongyan Quan, Zhiping Chen, Hongjuan Li, Shiguo Sun, Yongqian Xu
International journal of biological macromolecules 2024 SepThe concentration of urea in sweat serves as a valuable indicator of an individual's overall health. In this study, we present a novel hydrogel sensor (BAF-CPu), based on cellulose nanofiber and polyvinyl alcohol, designed to achieve non-invasive in situ and highly sensitive detection of urea in sweat by combining the dual-mode response of colorimetric and ratiometric fluorescence techniques. The bright red fluorescent gold‑copper bimetallic nanoclusters and green fluorescent fluorescein isothiocyanate-modified cellulose nanofibers endowed BAF-CPu with proportional fluorescence responsive properties. Under the catalytic action of urease, the hydrolysis of urea raises the pH, resulting in diminished red fluorescence along with enhanced green fluorescence, and the fluorescence color of BAF-CPu changes from red to green. Moreover, BAF-CPu hydrogel encapsulates pH-responsive bromothymol blue (BTB), which changes from yellow to blue in the presence of urea. Importantly, BAF-CPu absorbs sweat by adhering directly to the skin surface, avoiding the complicated sampling process and improving the maneuverability of the detection process. With both ratiometric fluorescence and colorimetric modes, BAF-CPu is not only able to detect sweat in situ, but also can reduce the interference of the complex sweat environment on the urea detection, and realize the high sensitivity detection of urea in sweat. Copyright © 2024 Elsevier B.V. All rights reserved.
Zongyan Quan, Zhiping Chen, Hongjuan Li, Shiguo Sun, Yongqian Xu. A hydrogel sensor based on cellulose nanofiber/polyvinyl alcohol with colorimetric-fluorescent bimodality for non-invasive detection of urea in sweat. International journal of biological macromolecules. 2024 Sep;276(Pt 1):133760
PMID: 39013510
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