Yuchen He, Benjamin M Faulkner, Meaghan A Roberti, Dana K Bassford, Cliff I Stains
Angewandte Chemie (International ed. in English) 2024 Oct 24Small GTPases comprise a superfamily of over 167 proteins in the human genome and are critical regulators of a variety of pathways including cell migration and proliferation. Despite the importance of these proteins in cell signaling, a standardized approach for controlling small GTPase activation within living cells is lacking. Herein, we report a split-protein-based approach to directly activate small GTPase signaling in living cells. Importantly, our fragmentation site can be applied across the small GTPase superfamily. We highlight the utility of these standardized parts by demonstrating the ability to directly modulate the activity of four different small GTPases with user-defined inputs, providing the first plug and play system for direct activation of small GTPases in living cells. © 2024 Wiley-VCH GmbH.
Yuchen He, Benjamin M Faulkner, Meaghan A Roberti, Dana K Bassford, Cliff I Stains. Standardized Parts for Activation of Small GTPase Signaling in Living Cells. Angewandte Chemie (International ed. in English). 2024 Oct 24;63(44):e202403499
PMID: 39058298
View Full Text