Clear Search sequence regions


  • breast cancer (1)
  • expressed gene (1)
  • genes C (1)
  • human (1)
  • MAGE (10)
  • MAGE A1 (1)
  • MAGE A6 (1)
  • MAGE A8 (1)
  • MAGE A9 (2)
  • MAGE B1 (1)
  • patients (2)
  • research (1)
  • rt pcr (1)
  • testes (1)
  • Sizes of these terms reflect their relevance to your search.

    Melanoma antigen gene (MAGE) families are cancer-testis genes that normally show expression in the testes. However, their expressions have been linked with various types of human cancers, including BC. Therefore, the primary purposes of the present research were to assess the expression of MAGE-A, -B, and -C genes in Saudi female patients with BC and determine their regulation via the epigenetic mechanism. Ten BC samples were analyzed for the expression levels of nine MAGE-A genes, six MAGE-B genes, and three MAGE-C genes using the RT-PCR technique. All 18 evaluated genes except for MAGE-A1, -A3, -A4, and -B5 showed weak band expressions in some BC specimens. MAGE-A6 and -B2 were expressed in 40 % of the BC tissue samples, and MAGE-A9, -A10, and -B6 were expressed in 30 %. The lowest expression levels were found for MAGE-A11, -B1, -B3, -B4, -C1, and -C2 in 10 % of the BC specimens and for MAGE-A9,--B2, and --C3 in 20 % of the samples. The most frequently expressed gene was MAGE-A8 (found in 70 % of the BC samples), which suggests that it may serve as - a marker for screening of BC. In vitro treatment, the 5-aza-2'-deoxycytidine agent led to a significant rise in mRNA expressions for all tested genes related to the MAGE-A family, except for MAGE-A10. By contrast, among the genes in the MAGE-B and -C families, only MAGE-B1 and -C2 exhibited detectable mRNA expression levels after treatment. © 2024 The Authors.

    Citation

    Ahmad M Almatrafi, Salman Alamery, Mikhlid H Almutairi. Expression pattern analysis of the MAGE family genes in breast cancer patients and hypomethylation activation in the MCF-7 cells. Heliyon. 2024 Jul 30;10(14):e34506


    PMID: 39082035

    View Full Text