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    Tert-butylhydroquinone (TBHQ) is easily overused or illegally added to edible oil and attracts a growing concern because of its cytotoxic, liver-damaging, and carcinogenic effects. Thus, a sensitive and intelligent point-of-care testing (iPOCT) method is developed to fulfill the on-site monitoring. This iPOCT method depended on a fluorescent immunochromatographic assay within 15 min. Under optimization, the limit of quantification (LOQ) was calculated as 0.03 μg mL-1. The iPOCT method provided a low limit of detection (LOD) of 0.02 μg mL-1, a wide linear range of 0.03-100 μg mL-1, and great selectivity. Recoveries by the spiking experiments ranged from 97.4% to 103.5% with relative standard deviations (RSDs) of 2.4%-4.9% in soybean, peanut, rapeseed, and corn oil samples. The results showed that the iPOCT method is highly consistent with the high-performance liquid chromatography (HPLC) method. Copyright © 2024 Elsevier Ltd. All rights reserved.

    Citation

    Dan Wang, Le Zhang, Jing Zeng, Shenling Wang, Wangwang Li, Yuanlong Sun, Wenqin Wu, Zhi-Quan Tian, Zhaowei Zhang. A sensitive intelligent point-of-care test method for tert-butylhydroquinone in edible oil via a test strip with a smartphone. Food chemistry. 2024 Dec 01;460(Pt 2):140625

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    PMID: 39089030

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