Development and evaluation of a radiolabelling agent for white blood cell scans.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2024 Oct

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Radiolabelled autologous leukocytes have been used for the clinical diagnosis of inflammation and infection. To develop a stable and efficient radiopharmaceutical for labelling leukocytes, we prepared a novel radioiodinated cell-penetrating peptide, 125I-TAT, using a bi-functional linker. 125I-TAT was stable for two days under three different temperature conditions of -20 °C, 4 °C, and 40 °C, with its radiochemical purity remaining over 99%. Iodinated TAT was non-toxic to leukocytes with an IC50 value of over 100 μM. The labelling efficiency of 125I-TAT using 1ｘ107 cells ranged from 27% to 53% when the three leukocyte cell lines were pre-treated with DMSO. This is comparable to the labelling efficiency recommended by the guideline for conventional labelling agents using 2ｘ108 cells. Radioiodinated cell-penetrating peptide may be an improved radiopharmaceutical for white blood cell scans by further optimization. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Byoung Soo Kim, Tae Hyun Choi. Development and evaluation of a radiolabelling agent for white blood cell scans. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 2024 Oct;212:111460