Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus.

Corinne Sailleau, Stéphan Zientara, Emmanuel Bréard

Methods in molecular biology (Clifton, N.J.) 2024

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Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene-beta-actin. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Corinne Sailleau, Stéphan Zientara, Emmanuel Bréard. Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus. Methods in molecular biology (Clifton, N.J.). 2024;2838:163-170