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Yeast two-hybrid (YTH) technology is a powerful tool for studying protein interactions and has been widely used in various fields of molecular biology, including the study of antiviral innate immunity. This chapter presents detailed information and experimental procedures for identifying virus-host protein interactions involved in immune regulation using yeast two-hybrid technology. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Sisi Xia, Xiaoping Huo, Chunfu Zheng, Jun Chen. Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity. Methods in molecular biology (Clifton, N.J.). 2025;2854:213-220

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PMID: 39192132

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