Didrik H Grevskott, Francisco Salvà-Serra, Edward R B Moore, Nachiket P Marathe
Journal of global antimicrobial resistance 2024 Aug 26The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15-carrying Escherichia coli strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the E. coli strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing. Isolation of E. coli was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two E. coli strains (3-338 and 5-325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing. The complete genome of strain 3-338 (GenBank accession no. CP130007-17) was assembled into a circular chromosome of 4.65 Mb and 10 plasmids (between 2 and 148 kb). Strain 5-325 (CP130018-27) exhibited a circular chromosome of 4.7 Mb and 9 plasmids (between 2 and 148 kb). Both strains carried an identical type 1/2 hybrid IncC plasmid (∼148 kb) harbouring multiple antibiotic resistance genes (ARGs), including blaCTX-M-15, blaOXA-1, blaTEM-1, qnrS1, sul2, aphA1, aacC2, mph(A) and floR. This plasmid also carried heavy metal resistance genes, such as chrA and arsR. Strain 5-325 carried an additional IncFIB plasmid (∼78 kb) harbouring additional ARGs, including blaTEM-1, qnrS1, tet(A), dfrA14, sul2, strA and strB. Our study shows the emergence of a CTX-M-15-carrying type 1/2 hybrid IncC plasmid in novel E. coli ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors. Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Didrik H Grevskott, Francisco Salvà-Serra, Edward R B Moore, Nachiket P Marathe. Escherichia coli novel sequence type 11873 harbours a new CTX-M-15-carrying multidrug resistance type 1/2 hybrid IncC plasmid. Journal of global antimicrobial resistance. 2024 Aug 26;39:109-113
PMID: 39197655
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