Hui-Zhen Tao, Wen-Bin He, Li Ding, Li Wen, Zhou Xu, Yun-Hui Cheng, Mao-Long Chen
Food chemistry 2024 Aug 30In this study, rice husk biochar was engineered with abundant iron ion sites to enhance the enrichment of antioxidant peptides from rice protein hydrolysates through metal-chelating interactions. The π-π interactions and metal ion chelation were identified as the primary mechanisms for the enrichment process. Through peptide sequencing, four peptides were identified: LKFL (P1: Leu-Lys-Phe-Leu), QLLF (P2: Gln-Leu-Leu-Phe), WLAYG (P3: Trp-Leu-Ala-Tyr-Gly), and HFCGG (P4: His-Phe-Cys-Gly-Gly). The vitro analysis and molecular docking revealed that peptides P1-P4 possessed remarkable scavenging ability against radicals and Fe2+ chelating ability. Notably, peptide P4 showed radical scavenging activity comparable to glutathione (GSH) against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) radicals. Cellular experiments further confirmed that peptide P4 effectively protected HepG2 cells from oxidative stress-induced damage. The modified rice husk biochar proved to be an effective means for enriching rice antioxidant peptides from protein hydrolysates.Copyright © 2024 Elsevier Ltd. All rights reserved.
Hui-Zhen Tao, Wen-Bin He, Li Ding, Li Wen, Zhou Xu, Yun-Hui Cheng, Mao-Long Chen. Enrichment of antioxidant peptide from rice protein hydrolysates via rice husk derived biochar. Food chemistry. 2024 Aug 30;463(Pt 1):141050141050
PMID: 39236384
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