The Arabidopsis U1 snRNP regulates mRNA 3'-end processing.

The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing alternative polyadenylation site selection in 3'-UTRs. Overall, our work provides a comprehensive view of U1 snRNP interactors and reveals novel functions in regulating mRNA 3'-end processing in Arabidopsis, laying the groundwork for understanding non-canonical functions of plant U1 snRNPs. © 2024. The Author(s).

Citation

Anchilie F Mangilet, Joachim Weber, Sandra Schüler, Manon Adler, Eneza Yoeli Mjema, Paula Heilmann, Angie Herold, Monique Renneberg, Luise Nagel, Irina Droste-Borel, Samuel Streicher, Thomas Schmutzer, Gregor Rot, Boris Macek, Cornelius Schmidtke, Sascha Laubinger. The Arabidopsis U1 snRNP regulates mRNA 3'-end processing. Nature plants. 2024 Oct;10(10):1514-1531

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PMID: 39313562

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