A Method for Analysis of Oxidized Phospholipids from Biological Samples Using Mass Spectrometry.

Mohan Ghorasaini, Daniela Costa, Victoria J Tyrrell, Majd Protty, Martin Giera, Valerie B O'Donnell

Methods in molecular biology (Clifton, N.J.) 2025

filter terms:

Oxidized phospholipids (oxPLs) are generated during innate immunity and inflammation, where they play a variety of biological roles, including regulation of autoimmunity and coagulation. Some are generated by enzymatic reactions, leading to stereo- and regiospecificity, while many others can be formed through nonenzymatic oxidation and truncation and can be used as biomarkers of oxidative stress. Mass spectrometry methods have been developed over many years for oxPL analysis, which can provide robust estimations of molecular species and amounts, where standards are available. Here we present a method used for the analysis of enzymatically-generated oxPL (eoxPL), which allows quantification of mono-hydroxy oxylipin-containing species. We also show profiling of many other partially characterized structures in tissue samples and provide typical chromatograms obtained. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Mohan Ghorasaini, Daniela Costa, Victoria J Tyrrell, Majd Protty, Martin Giera, Valerie B O'Donnell. A Method for Analysis of Oxidized Phospholipids from Biological Samples Using Mass Spectrometry. Methods in molecular biology (Clifton, N.J.). 2025;2855:155-169