Sphingolipid Analysis in Clinical Research.

Jeongah Oh, Bo Burla, Sneha Muralidharan, Markus R Wenk, Federico Torta

Methods in molecular biology (Clifton, N.J.) 2025

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Sphingolipids are the most diverse class of lipids due to the numerous variations in their structural components. This diversity is also reflected in their extremely different functions. Sphingolipids are not only constituents of cell membranes but have emerged as key signaling molecules involved in a variety of cellular functions, such as cell growth and differentiation, proliferation and apoptotic cell death. Lipidomic analyses in clinical research have identified pathways and products of sphingolipid metabolism that are altered in several human pathologies. In this article, we describe how to properly design a lipidomic experiment in clinical research, how to handle plasma and serum samples for this purpose, and how to measure sphingolipids using liquid chromatography-mass spectrometry. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Jeongah Oh, Bo Burla, Sneha Muralidharan, Markus R Wenk, Federico Torta. Sphingolipid Analysis in Clinical Research. Methods in molecular biology (Clifton, N.J.). 2025;2855:225-268