Clear Search sequence regions


filter terms:
Sizes of these terms reflect their relevance to your search.

Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein levels in murine skeletal muscle. We describe steps for tibialis anterior dissection, formaldehyde fixation, tissue chopper cutting, and hybridization chain reaction (HCR) detection and amplification. We then detail procedures for immunostaining, tissue clearing, and imaging. This protocol is easily adaptable to other tissues.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Paola Pisterzi, Clara Martinez Mir, Ouafa Dahri, Isabel de Poorter, Sandra Batlles Parera, Milica Dostanić, Massimo Mastrangeli, Christine Mummery, Niels Geijsen, Fanny Sage. Spatial analysis of transcript and protein levels in skeletal muscle. STAR protocols. 2024 Oct 09;5(4):103378103378


PMID: 39388355

View Full Text