Activation of mouse skin mast cells and cutaneous afferent C-fiber subtypes by bee venom.

In mammals, many Hymenopteran stings are characterized by pain, redness, and swelling - three manifestations consistent with nociceptive nerve fiber activation. The effect of a Western honeybee (Apis mellifera) venom on the activation of sensory C-fibers in mouse skin was studied using an innervated isolated mouse skin preparation that allows for intra-arterial delivery of chemicals to the nerve terminals in the skin. Our data show that honeybee venom stimulated mouse cutaneous nociceptive-like C-fibers, with an intensity (action potential discharge frequency) similar to that seen with a maximally-effective concentration of capsaicin. The venom had a stronger effect on chloroquine-sensitive C-fibers compared to chloroquine-insensitive C-fibers, an effect that was recapitulated with a wasp (Vespula spp.) venom. Blocking TRPV1 and TRPA1 channels did not influence the honeybee venom-induced C-fiber activation. The effect of the venoms on chloroquine-sensitive and -insensitive subpopulation of C-fiber terminals was mimicked by melittin but not apamin; two of peptide venom components. Chloroquine-sensitive C-fibers are stimulated as a consequence of mast cell activation. Melittin degranulated mast cells in mouse skin by a non-IgE and non-MrgprB2 mechanism, and this may explain the stronger activation of the chloroquine-sensitive C-fibers. Copyright © 2024 Elsevier B.V. All rights reserved.

Citation

Danica Jurcakova, Fei Ru, Renata Pecova, Bradley J Undem. Activation of mouse skin mast cells and cutaneous afferent C-fiber subtypes by bee venom. Neuroscience letters. 2025 Jan 10;845:138061

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PMID: 39603444

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