Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.

Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Joan Antoni Soler, Anupam Singh, Marino Zerial, Shashi Thutupalli. Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS. Methods in molecular biology (Clifton, N.J.). 2025;2881:87-115

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PMID: 39704939

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