Bacterial surface informatics reliant on multi-mechanism simultaneous detection for Salmonella typhimurium and Staphylococcus aureus.

Fully excavating and utilizing the rich information presented on bacterial surfaces can open innovative solutions for the multi-mechanism detection of food-borne pathogens. In this work, a colorimetric-fluorescence dual-signal lateral flow immunoassay was used to establish a simultaneous detection strategy integrating five physical, chemical, and biometric combining mechanisms for Salmonella typhimurium (S. typhimurium) and Staphylococcus aureus (S. aureus). To improve sensitivity, a layer-by-layer design of aggregation-induced emission nanosphere@ polydopamine@ platinum (AIENS@PDA@Pt) nanostructure with excellent colorimetric-fluorescence signal, abundant recognition sites, and rough surface was employed as a signal tag. Under optimal conditions, the colorimetric detection limit of the multi-mechanism reliant simultaneous detection LFIA (multi-MRSD LFIA) for S. typhimurium and S. aureus is 2 × 104 and 5 × 104 cfu·mL-1, respectively, and the fluorescence detection limit is 500 cfu·mL-1 for both target bacteria. This study introduces a novel approach for multi-target, multi-mechanism, and multi-signal output detection of bacteria, providing a significant direction for pathogen research. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Xuechi Yin, Jiayi Zhang, Yuanyuan Cheng, Yangyang Lu, Qiaoying Wu, Leina Dou, Yanru Wang, Jianlong Wang, Daohong Zhang. Bacterial surface informatics reliant on multi-mechanism simultaneous detection for Salmonella typhimurium and Staphylococcus aureus. Food chemistry. 2025 Mar 30;469:142579

Mesh Tags

PMID: 39724704

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