Intracellular protein ligation and polyprotein synthesis using an asparaginyl endopeptidase core.

An intracellular protein ligation system using a truncated variant of OaAEP1 (OaAEP1 core) to ligate and polymerize Ig domains in E. coli is developed, enabling real-time polyprotein synthesis without enzyme activation and external ligation. This approach yields functional, mechanically stable polyproteins and expands AEP applications in synthetic biology and biomaterials.

Citation

Renming Liu, Jun Qiu, Yifen Huang, Ziyi Wang, Peng Zheng. Intracellular protein ligation and polyprotein synthesis using an asparaginyl endopeptidase core. Chemical communications (Cambridge, England). 2025 Mar 11;61(22):4355-4358

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PMID: 39981834

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