Evaluation of Constitutive or Induced Activation State of the VLA-4 Integrin in Human and Murine Samples.

The integrin heterodimer CD49d/CD29 (a.k.a. Very Late Antigen-4, VLA-4) mediates cell-cell and cell-matrix interaction through binding to its specific ligands. VLA-4 can be present on the cell surface at different conformation states that affect the binding affinity for the ligands. In chronic lymphocytic leukemia (CLL), higher VLA-4 levels have been demonstrated to be associated with a worse prognosis both in the chemo-immunotherapy era and in the BCR inhibitor setting, in keeping with the role of VLA-4 as a key molecule favoring CLL cell localization in protective niches of bone marrow and lymph nodes. Here, we describe functional flow cytometry-based methods to assess the activation state of the VLA-4 integrin, applicable in both human and murine settings, as well as in fresh or thawed samples. A specific "R" script for analyzing flow cytometry data is also provided. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Erika Tissino, Antonella Zucchetto, Federico Pozzo, Tanja Nicole Hartmann, Valter Gattei. Evaluation of Constitutive or Induced Activation State of the VLA-4 Integrin in Human and Murine Samples. Methods in molecular biology (Clifton, N.J.). 2025;2909:45-60

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PMID: 40029514

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