Diuretic binding with biological membranes studied by means of a fluorescent probe].

The binding of furosemide, novurit and ethacrynic acid with human serum albumin, phospholipid liposomes, mitochondria and plasmatic membranes of rat kidneys was studied by the fluorescent probe 1-anilino naphthalene-8-sulfonate (1,8-ANS) method. It is shown that ethacrynic acid has high associative constants with serum albumin molecule and plasmatic membranes. Novurit has the greatest affinity with rat kidney mitochondria. Furosemide does not bind with plasmatic membranes and has very low constants of association with mitochondria and phospholipid liposomes.

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O V Samokrutova. Diuretic binding with biological membranes studied by means of a fluorescent probe]. Farmakologiia i toksikologiia. 1985 Nov-Dec;48(6):112-6

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PMID: 4085623

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