Presence of elongation factor 1 in nuclei and nucleoli of rat liver.

M S Rao, L I Rothblum, H Busch

Cell biology international reports 1978 Jan

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Purified rat liver nuclei contain 11% of the total cellular translation elongation factor 1 activity. Ninety percent of the nuclear EF-1 activity was in the nucleoplasm and 10% was nucleolar. The specific activities of the nuclear and nucleolar EF-1 were 2 to 3 times higher, respectively, than EF-1 activity of the liver homogenate. The presence of EF-1 in the purified nuclei did not result from cytoplasmic contamination since only 0.14% of the cellular lactate dehydrogenase was present in the nuclei. These results provide the first evidence for the presence in the cell nucleus of translational factors of protein synthesis.

Citation

M S Rao, L I Rothblum, H Busch. Presence of elongation factor 1 in nuclei and nucleoli of rat liver. Cell biology international reports. 1978 Jan;2(1):25-32