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We tried to see whether it was possible to use Bactofen to decontaminate sputum for culture of Mycobacterium tuberculosis. Bactofen showed very little bactericidal action on tubercle bacilli, lower than Desogen, and selectively destroyed many contaminants, more actively than benzalkonium chloride. Digested sputum decontaminated with 0.2% Bactofen and then applied to culture media without centrifugation gave good results in growth of colonies.


P Carpi Torelli, E Tortoli. The use of dodecyl-di(beta-oxyethyl) benzylammonium chloride (Bactofen) as a decontaminant in the cultural examination for detection of the Koch bacillus in sputum]. Quaderni Sclavo di diagnostica clinica e di laboratorio. 1980 Jun;16(2):196-205

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PMID: 6787655

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