Lack of metabolism of 2,6-dimethyldinitrosopiperazine by microsomes and postmicrosomal supernatant prepared from the rat esophagus and non-glandular stomach.

R A Scanlan, J G Farrelly, L I Hecker, W Lijinsky

Cancer letters 1980 Oct

filter terms:

Microsomes and postmicrosomal supernatant were prepared from the esophagus and non-grandular stomach of rats. Using these fractions, we could not demonstrate in vitro metabolism of 2,6-dimethyldinitrosopiperazine (DMDNP), a potent esophageal and non-grandular stomach carcinogen in rats. The esophageal and non-grandular stomach fractions did metabolize N-nitrosopyrrolidine (NPYR) to a small extent, and liver microsomes and postmicrosomal supernatant metabolized both nitrosamines to a similar extent. Therefore, we advise caution in the interpretation of metabolic studies using 'target' and 'non-target' organs as indicative of activation of compounds to proximate carcinogens.

Citation

R A Scanlan, J G Farrelly, L I Hecker, W Lijinsky. Lack of metabolism of 2,6-dimethyldinitrosopiperazine by microsomes and postmicrosomal supernatant prepared from the rat esophagus and non-glandular stomach. Cancer letters. 1980 Oct;10(4):293-9