Co-amplification of c-myc/pvt-1 in immortalized mouse B-lymphocytic cell lines results in a novel pvt-1/AJ-1 transcript.

In a series of mouse pvt-1 cDNA clones prepared from an immortalized B-cell line that contains an amplified c-myc/pvt-1 region, we have identified a unique cDNA, AJ-I, which contains 57 bp of pvt-1 sequence (pvt-1a) spliced to a novel sequence of 2.1 kb. We report here that this 3' segment (termed AJ-IX) maps more than 60 kb telomeric to pvt-1a and is encoded by a novel locus (AJ-I) on mouse chromosome 15. The AJ-IX probe detects a transcript that is expressed only in normal mouse brain and testis. Several mast-cell tumors, Ly-I+ B-lymphocytic cell lines and a neuroblastoma also display abundant levels of AJ-IX-specific mRNA. However, splicing of pvt-1a to AJ-IX is found exclusively in Ly-I+ B-cell lines that contain amplified c-myc/pvt-1. We conclude that some features in the generation of this amplicon facilitates the synthesis of a pvt-1/AJ-IX chimeric mRNA that may play a role in immortalization of these Ly-I+ B-cell lines.

Citation

K Huppi, D Siwarski, J D Shaughnessy, J F Mushinski. Co-amplification of c-myc/pvt-1 in immortalized mouse B-lymphocytic cell lines results in a novel pvt-1/AJ-1 transcript. International journal of cancer. 1993 Feb 1;53(3):493-8

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PMID: 8094075

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