Isolation and structure of woodorien, a new glucoside having antiviral activity, from Woodwardia orientalis.

H X Xu, S Kadota, M Kurokawa, K Shiraki, T Matsumoto, T Namba

Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.

Chemical & pharmaceutical bulletin 1993 Oct

filter terms:

Hot aqueous and methanol extracts of the rhizomes of Woodwardia orientalis were tested for their in vitro antiviral activity against herpes simplex virus type 1 (HSV-1), poliovirus type 1, and measles virus by plaque reduction assay. The aqueous extract of W. orientalis reduced the plaque forming ability of HSV-1 and poliovirus more strongly than did the methanol extract. By bioassay-directed fractionation of the aqueous extract, a new glucoside, woodorien (1), along with five known compounds were isolated from an EtOAc-soluble fraction that had antiviral activity. The structures of these compounds were determined by the use of two dimensional (2D) NMR techniques (1H-1H correlation spectroscopy (COSY), 1H-13C COSY and heteronuclear multiple-bond multiple-quantum coherence (HMBC)). Woodorien (1) was the most potent inhibitor against HSV-1 among the isolated compounds.

Citation

H X Xu, S Kadota, M Kurokawa, K Shiraki, T Matsumoto, T Namba. Isolation and structure of woodorien, a new glucoside having antiviral activity, from Woodwardia orientalis. Chemical & pharmaceutical bulletin. 1993 Oct;41(10):1803-6