Comparison of different antibody-conjugate derivatives for the development of a sensitive and specific progesterone assay.

G Hatzidakis, A Stefanakis, E Krambovitis

Department of Immunology and Diagnostics, Institute of Molecular Biology and Biotechnology, Crete, Greece.

Journal of reproduction and fertility 1993 Mar

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Antibodies raised against progesterone with hormone-carrier protein bridges placed at four different carbon positions were used to compare the sensitivity and specificity of homologous and heterologous enzyme-hormone conjugates. All heterologous assays were at least twice as sensitive as the corresponding homologous assays. The best results were obtained by using antibodies against 7 alpha-carboxyethyl-thioether-progesterone with 6 beta-hemisuccinate-progesterone conjugate (or hemimaleate). The sensitivity with human sera was 0.25 ng ml-1 and, the highest crossreaction 10% with 5 beta-pregnane-3,20-dione, and reproducibility, recovery and accuracy were satisfactory. The correlation coefficient with radioimmunoassay in 103 human sera tested was r = 0.915. The assay was successfully applied for the diagnosis of pregnancy in dairy cattle.

Citation

G Hatzidakis, A Stefanakis, E Krambovitis. Comparison of different antibody-conjugate derivatives for the development of a sensitive and specific progesterone assay. Journal of reproduction and fertility. 1993 Mar;97(2):557-61