Cytochrome c oxidase subunit VIIa liver isoform. Characterization and identification of promoter elements in the bovine gene.

R S Seelan, L Gopalakrishnan, R C Scarpulla, L I Grossman

The Journal of biological chemistry 1996 Jan 26

filter terms:

Cytochrome c oxidase subunit VIIa is specified by two nuclear genes, one (COX7AH) producing a heart/muscle-specific isoform and the other (COX7AL) a form expressed in all tissues. We have isolated both genes to examine their transcriptional regulation. Here, we characterize the core promoter of COX7AL and show that a 92-base pair region flanking the 5'-end promotes most of the activity of this gene. The 92-bp basal promoter contains sites for the nuclear respiratory factors NRF-1 and NRF-2, which have been shown to contribute to the transcription of a number of nuclear genes involved in mitochondrial respiratory activity, and also at least four Sp1 motifs. We show that both the NRF-1 and NRF-2 binding sites are functional in COX7AL and present evidence suggesting that interaction between the NRF-1 site and an upstream element contributes to expression.

Citation

R S Seelan, L Gopalakrishnan, R C Scarpulla, L I Grossman. Cytochrome c oxidase subunit VIIa liver isoform. Characterization and identification of promoter elements in the bovine gene. The Journal of biological chemistry. 1996 Jan 26;271(4):2112-20