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Photoaffinity probes PPDA and PPTD, which have diazoacetyl and trifluorodiazopropionyl group at C-13 position in phorbol, respectively, were synthesized. Photoaffinity labeling of protein kinase C isozymes by both the probes resulted in specific cross-linking.


K Uotsu, M Sodeoka, M Shibasaki. Photoaffinity labeling of PKC isozymes by phorbol ester derivatives. Bioorganic & medicinal chemistry. 1998 Jul;6(7):1117-26

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PMID: 9730248

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