Cloning of a novel cDNA expressed during the early stages of fracture healing.

M Hadjiargyrou, M F Halsey, W Ahrens, E P Rightmire, K J McLeod, C T Rubin

Program in Biomedical Engineering, State University of New York at Stony Brook 11794-8181, USA. mhadjiargyro@ccmail.sunysb.edu

Biochemical and biophysical research communications 1998 Aug 28

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Using differential mRNA display (DD-PCR), a novel cDNA, FxC1 (Fracture Callus 1) was isolated from the early stages of a healing fractured femur. Utilizing 5' RACE PCR, a 598-bp full-length cDNA was obtained for FxC1 that contains an open reading frame (ORF) of 243 bp, encoding for an 80 amino acid protein. Within this ORF, a leucine zipper motif was present. In vitro transcription/translation of the full-length cDNA generated the expected 9-kDa protein. Northern analysis reveals that this gene is expressed in calluses harvested from post-fracture day 5, 7 and 10, as well as in several other tissues and bone-derived cell lines. During the differentiation of MC3T3 cells along the osteoblast lineage, FxC1 expression increases 3- to 4-fold during the production and deposition of matrix proteins, suggesting a possible role for this protein in cell differentiation.

Citation

M Hadjiargyrou, M F Halsey, W Ahrens, E P Rightmire, K J McLeod, C T Rubin. Cloning of a novel cDNA expressed during the early stages of fracture healing. Biochemical and biophysical research communications. 1998 Aug 28;249(3):879-84