Terbium chelates for fluorescence immunoassays.

S V Beltyukova, A V Egorova

A.V. Bogatsky Physico-Chemical Institute of Academy of Sciences of Ukraine, Odessa.

Journal of pharmaceutical and biomedical analysis 1998 Oct

filter terms:

The sensitization of terbium(III) ion luminescence in the presence of 1-ethyl-14-dihydro-6,7-methylenedioxy-4-oxoquinoline-3-carboxylic (oxolinic) acid was studied. The terbium label is bound to the antibody with diethylenetriamine-pentaacetic acid anhydride (DTPAA). Optimum luminescence intensity is observed at pH 7.5 and the luminescence significantly increases in the presence of cationic surfactant, cetyltrimethylammonium bromide. The sensitivity of Tb(III) detection is 5 x 10(14) mol l(-1). This luminescence system is proposed for time-resolved fluoroimmunoassay.

Citation

S V Beltyukova, A V Egorova. Terbium chelates for fluorescence immunoassays. Journal of pharmaceutical and biomedical analysis. 1998 Oct;18(1-2):267-70