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QuickView for MPN domain (biogroup)
- Description:
- The MPN (Mpr1, Pad1 N-terminal) domain (also known as the Mov34, JAB, PAD-1,or JAMM (JAB1/MPN/Mov34 metalloenzyme) domain) is a widespread 120 amino acidprotein module found in archaea, bacteria and eukaryotes. In eukaryotes theMPN domain is found in subunits of several multiprotein complexes includingthe proteasome, whereas in eubacteria and archaea, the MPN domain is usuallyfound in single domain proteins.Within the MPN domain super-family, two main subclasses have beencharacterized: the MPN+ and MPN- domain-containing proteins. MPN+ domain-containing proteins are classified as metalloenzymes responsible forisopeptidase activity. These proteins contain a conserved glutamate (E) and aJAMM (Jab1/MPN/Mov34 metalloenzyme) motif, typically consisting of a canonicalsequence (H-x-H-x-S-x-D) and coordinating a zinc ion. The E and JAMM motif specify a catalytic centre essential for selective hydrolysis of linkages, contained between ubiquitin/ubiquitin-like proteins and target proteins or between ubiquitin monomers within a polymeric chain. MPN- domains are recognizable by the absence of essential Zn(2+)-coordinating residues that are required for catalytic function. In protein complexes, an MPN+ domain can associate with MPN- domains for purposes that are not well understood.The MPN domain is composed of a five-stranded mixed beta-sheet with strandorder 21345 sanwiched between two alpha-helices. There is a second smallerthree-stranded parallel beta-sheet formed by residues at the N and C terminiand in the loop between the first two strands of the main sheet. MPN domains often form part of larger proteins. The N and Ctermini of the structure are close in space, allowing it to be easily insertedinto a multidomain protein. The histidine and aspartate residues of the JAMMmotif coordinate a zinc ion.Some proteins known to contain a MPN domain are listed below:Eukaryotic COP9 signalosome complex subunit 5 (CSN5).Eukaryotic COP9 signalosome complex subunit 6 (CSN6).Eukaryotic 26S proteasome regulatory subunit RPN11.Eukaryotic 26S proteasome non-ATPase subunit 7 (PSMD7), also known as Mov34.Eukaryotic AMSH (associated molecule with the SH3 domain of STAM) proteins, zinc metalloproteases that specifically cleave ubiquitin chains.Eukaryotic pre-mRNA-splicing factor 8 (PRP8), a component of the U5 small nuclear RNA-protein (snRNP) complex.Eukaryotic translation initiation factor 3 (eIF3) subunits f and h (also known as subunits p47 and p40, respectively).Animal BRCC36 family proteins, Lys-63-specific deubiquitinases.Animal BRCA1-A complex subunit Abraxas.Animal BRISC complex subunit Abro1.Bacterial UPF0758 protein.Bacteriophage lambda tail assembly protein 'K' (vtak).
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