The product of the ABC half-transporter gene ABCG2 (BCRP/MXR/ABCP) is expressed in the plasma membrane.

E Rocchi, A Khodjakov, E L Volk, C H Yang, T Litman, S E Bates, E Schneider

Wadsworth Center, New York State Department of Health, Albany, New York 12201, USA.

Biochemical and biophysical research communications 2000 Apr 29

filter terms:

The products of the ABC gene family can be generally classified as either full-transporters of half-transporters. Full-transporters are expressed in the plasma membrane, whereas half-transporters are usually found in intracellular membranes. Recently, an ABC half-transporter, the ABCG2 gene product Breast Cancer/Mitoxantrone Resistance Protein (BCRP/MXR), has been shown to cause mitoxantrone and topotecan resistance. The purpose of this study was to determine the expression and the intracellular localization of this protein in various drug-resistant cell lines. BCRP/MXR expression was determined by Western blot and immunohistochemistry. This protein is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane, instead of to intracellular membranes as seen with all other known half-transporters. Therefore, BCRP/MXR is unique among the ABC half-transporters by being localized to the plasma membrane. Copyright 2000 Academic Press.

Citation

E Rocchi, A Khodjakov, E L Volk, C H Yang, T Litman, S E Bates, E Schneider. The product of the ABC half-transporter gene ABCG2 (BCRP/MXR/ABCP) is expressed in the plasma membrane. Biochemical and biophysical research communications. 2000 Apr 29;271(1):42-6