Preliminary study of apoptosis of human hepatocarcinoma cell line HepG2 induced by topotecan].

Jun Zhang, Shu-ping Pu, Yu-jian Zhou

Ai zheng = Aizheng = Chinese journal of cancer 2002 Dec

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Topotecan, a semisynthetic water-soluble derivative of camptothecin, is a potent inhibitor of DNA topoisomerase I and cell cycle specific antitumor agent; The incidence rate of hepatocarcinoma ranks the third in all types of malignant tumor in China. The clinical curative effect of the present treatments and drugs for hepatocarcinoma are not so satisfactory. The inducing effect of topotecan on apoptosis of hepatocarcinoma cell line HepG2 and its cytotoxicity on HepG2 were studied in this paper. Microculture tetrazolium assay (MTT), HE staining, transmission electron microscope (TEM), flow cytometer (FCM) and immunohistochemistry were employed to determine and observe the apoptosis of HepG2 induced by topotecan and its lethal action on HepG2. Topotecan killed HepG2 cell by inducing apoptosis. Topotecan had strong anti-HepG2 activity in vitro, suggesting distinct dose- and time-dependent relationship. The drug IC50 was about 95 micrograms/L. Topotecan block HepG2 in S-phase, then further initiate apoptosis program. The morphologic characteristic of apoptosis showed that it is a typical interphase apoptosis process: the nuclei showed chromatin pycnosis, and clustered on the inner border of karyotheca, cytoplasm condensed, many vacuoles occurred in it. The expression of bcl-2 were not affected in the course of HepG2 apoptosis induced by topotecon (P > 0.05). Topotecan had significant cytotoxicity on HepG2 by inducing apoptosis.

Citation

Jun Zhang, Shu-ping Pu, Yu-jian Zhou. Preliminary study of apoptosis of human hepatocarcinoma cell line HepG2 induced by topotecan]. Ai zheng = Aizheng = Chinese journal of cancer. 2002 Dec;21(12):1305-9